Sr. No.

Criteria

Minimum

Maximum

Optimum

1

Amplicon size

150

1000

-

2

GC Clamps

0

-

-

3

Primer Size

18

22

20

4

Tm

55

61

59

5

Content G/C

45

50

-

6

Region scanned

Auto

Auto

-

7

End Stability

250

-

-