Sr. No. | Criteria | Minimum | Maximum | Optimum |
1 | Amplicon size | 150 | 1000 | - |
2 | GC Clamps | 0 | - | - |
3 | Primer Size | 18 | 22 | 20 |
4 | Tm | 55 | 61 | 59 |
5 | Content G/C | 45 | 50 | - |
6 | Region scanned | Auto | Auto | - |
7 | End Stability | 250 | - | - |